Abstract: MPEG Dynamic Adaptive Streaming over HTTP (DASH) is a new streaming standard that has been recently ratified as an international standard (IS). In comparison to other streaming systems, e.g., HTTP progressive download, DASH is able to handle varying bandwidth conditions providing smooth streaming. Furthermore, it enables NAT and Firewall traversal, flexible and scalable deployment as well as reduced infrastructure costs due to the reuse of existing Internet infrastructure components, e.g., proxies, caches, and Content Distribution Networks (CDN). Recently, the Hypertext Transfer Protocol Bis (httpbis) working group of the IETF has officially started the development of HTTP 2.0. Initially three major proposals have been submitted to the IETF i.e., Googles' SPDY, Microsofts' HTTP Speed+Mobility and Network-Friendly HTTP Upgrade, but SPDY has been chosen as working draft for HTTP 2.0. In this paper we implemented MPEG-DASH over HTTP 2.0 (i.e., SPDY), demonstrating its potential benefits and drawbacks. Moreover, several experimental evaluations have been performed that compare HTTP 2.0 with HTTP 1.1 and HTTP 1.0 in the context of DASH. In particular, the protocol overhead, the performance for different round trip times, and DASH with HTTP 2.0 in a lab test scenario has been evaluated in detail.